The ROIs included PD-L1 negative muscle (Fig. 4g, h; ‘M’), adipose tissue (Fig. 4g; ‘AT’), and blood vessels (Fig. 4g–i; ‘BV’) along with tumor cells that are either PD-L1 positive (Fig. 4h, i; ‘T’) or negative (Fig. 4i; ‘*’). Here, CD274 is linked to neoplasm.