TFEB and peripheral arterial disease: Further experiments revealed that exercise pretreatment improved stroke prognosis by activating the AMP-activated protein kinase pathway, leading to transcription factor EB nuclear translocation.[47] Exercise may have increased perfusion, arteriole density, and muscle regeneration in the ischemic hindlimb regeneration by inhibiting M1 macrophage polarization and inflammation in ischemic muscles and ultimately increased the maximum running distance and maximum running time of PAD model mice.