The human MHC non-restricted cytotoxic CD8+ T cell leukemia line TALL-104 cells were cultured with or without the presence of 10 nM or 100 nM 1,25(OH)2D3 in normal 10 mM glucose-containing medium, and medium without 1,25(OH)2D3 and containing 5 mM glucose was used as a negative control. The gene discussed is CD8A; the disease is T-cell leukemia.