Factorial analysis showed that GDM increased the protein expression levels of PI3K (p = 0.002) and Akt (p < 0.001) but had no effect on the protein expression levels of PIP5K1A and NOX1 in the livers of weaning female offspring; Se deficiency in maternal mice diminished the Akt protein expression level (p < 0.001) but failed to exert any effect on the protein expression levels of PI3K, PIP5K1A, and NOX1. Here, PIP5K1A is linked to gestational diabetes.