FASN and steatosis: To verify that oleic acid-induced steatosis in HepG2 cells is accompanied by changes in key enzymes of lipid metabolism, the mRNA levels of enzymes of lipid metabolism were detected using RT-qPCR, including acetyl-CoA carboxylase (ACC), carnitine palmitoyltransferase-1 (CPT-1), fatty acid synthase (FAS), glycerol-3-phosphate acyltransferases (GPAT), 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoR) and hormone-sensitive lipase (HSL).