Further Dunnett test analysis proved that group V showed highly significant (p < 0.01) improvement in insulin levels compared to group I. Further, group III and group IV showed significant (p < 0.00) improvement in insulin levels compared to group I. Group II’s insulin levels were significantly (p < 0.00) lower due to obesity in the animals compared to group I as it is mentioned in Figure 2. Here, INS is linked to obesity due to melanocortin 4 receptor deficiency.