Our results showed that paxillin Y31F overexpression inhibited the TGF-β1-induced migration (Figure 7A,C,D) and invasion (Figure 7B–D) of both MDA-MB-231 and MCF7 cells, suggesting that paxillin Tyr31 phosphorylation plays an important role in the migration and invasion of breast cancer cells. The gene discussed is TGFB1; the disease is breast carcinoma.