Later, Manczak et al. [8,78] using immunoblotting, fractionation with digitonin, immunofluorescence, and electron microscopy techniques, found a relationship between mutant APP derivatives and mitochondria in brain slices of Tg2576 mice—a mouse model overexpressing a mutant form of APP (isoform 695) with the Swedish mutation (KM670/671NL), causing amyloid plaques and progressive cognitive deficits—and in mouse neuroblastoma cells expressing mutant human APP. Here, APP is linked to neuroblastoma.