Immunofluorescence staining was performed on cells derived from 13 patients for the presence of cancer-associated fibroblasts (CAFs) markers (fibroblasts activation protein—FAP, platelet-derived growth factor receptor alpha—PDGFRα), epithelial to mesenchymal transition (EMT) marker (Snail, vimentin), CSCs markers (ALDH1, c-kit), pluripotency-related markers (Oct4, Sox2, Nanog). The gene discussed is NANOG; the disease is cancer.