NPVF and Infertility: Importantly, female mice exhibiting RFRP neuron ablation or inhibition did not exhibit the stress-induced suppression in pulsatile LH secretion observed in control females, and Rfrp gene silencing using targeted shRNA alleviated various stress-induced infertility measures in female rats [32], suggesting that RFRP neurons play a role in mediating the suppressive effects of stress on the neuroendocrine reproductive axis [31] (discussed in greater detail later in this review).