In contrast, for EpCAM and CD133, a stronger reduction in the proportion of cells with these markers was observed after UA and IR treatment in the 3D culture of HCT116 than in the 2D culture, and the number of EpCAM-positive cells reached about 55–60% in the spherical culture of the colon cancer cells, while CD133-positive cells accounted for less than 3% of the cells. The gene discussed is PROM1; the disease is malignant colon neoplasm.