Methylation analysis of 44 patients using next-generation sequencing identified five proteins significant in osteoporosis: Zinc Finger Protein 267 (ZNF267), Actin Binding LIM Protein Family Member 2 (ABLIM2), Ras Homolog Family Member J (RHOJ), Cyclin-Dependent Kinase-Like 5 (CDKL5), and Programmed Cell Death 1 (PDCD1) [105,106]. This evidence concerns the gene PDCD1 and osteoporosis.