Next, we measured the levels of mucin secretion and the expression of the membrane water channel in the mid colon of the Urd- and AEtLP-treated C3 KO mice to determine whether the laxative effects of Urd and AEtLP in C3-deficiency-induced constipation were accompanied by an alteration in the mucin secretion ability and water retention capacity. This evidence concerns the gene MUC5AC and constipation disorder.