On the other hand, KCa1.1 protein expression and channel activity were increased by 3D spheroid formation in osteosarcoma MG-63, chondrosarcoma SW-1353, and prostate cancer LNCaP cells, mediating the down-regulation of the E3 ubiquitin ligase, FBXW7 [4,5,6]. The gene discussed is KCNMA1; the disease is osteosarcoma.