TLR4 and Sepsis: Furthermore, hydrophobic interactions were observed between the C ring of rhamnetin and Leu61 and Ile63, with one between the B ring and Ile94 and Ile117, as shown in the two-dimensional plot of Figure 4D. Therefore, rhamnetin may exert anti-inflammatory effects on CRAB-induced sepsis by inhibiting TLR4 activity through a direct interaction with MD-2.