Transgenic zebrafish expressing myogenic factor 5 (myf5)-GFP, myogenin-H2B-mRFP, and mylpfa-lyn-Cyan were able to show the sub-population of developed ERMS tumors, indicating GFP fluorescence for tumor propagating cells (TPCs) and cyan fluorescence for differentiated cells. This evidence concerns the gene MYF5 and embryonal rhabdomyosarcoma.