Overall, on the basis of the combined observation of experiments performed with mutationally restricted Hsp90α mutants, we concluded that while both conformations of Hsp90 are capable of binding to Collagen-1, it is exclusively the open conformation of Hsp90 that aligns Collagen-1 fibers, leading to a subsequent increase in the invasiveness of breast cancer cells through the Collagen-1 matrix. This evidence concerns the gene HSP90AA1 and breast carcinoma.