PHB1 and cancer: Under the guidance of bioinformatic analysis for pan-cancer, we have successfully constructed the model of higher PHB breast cancer cell with transient transfection, and explored the biological functions that inhibited cell proliferation, arrested cell cycle in the S/G2 phase, decreased cell viability, and invasive ability of MCF-7, and accelerated cell apoptosis in vitro, this study indicate PHB regulates breast cancer cell survival and predicts biomarker function.