We used the sections (normal tissue adjacent to renal Wilms' tumor) that were not incubated with ANLN primary antibody and incubated with the corresponding fluorescent secondary antibody as the control. Compared with the control group (normal tissue adjacent to renal Wilms' tumor), the colocalization of ANLN and WT1 was rarely observed in the biopsy kidney tissue of proband A-II-1 (Fig. 1c). The gene discussed is WT1; the disease is kidney Wilms tumor.