As a result, ACAT1, ADH4 and ECI1 were even more down-regulated in HBV-positive HCC tumor tissue (Fig. 7A), and ACAT1, ADH4 and ACSL3 were down-regulated in HepG2.2.15 to a higher degree (Fig. 7B). The gene discussed is ACAT1; the disease is hepatocellular carcinoma.