PRKDC and neuroblastoma: Thus, we performed western blots to examine the induction of phosphorylation at ser-2056 (pPRKDC) following doxorubicin treatment in the neuroblastoma BE2C and GIMEN cell lines (0.4 μM in BE2C, 0.03 μM in GIMEN – the approximate IC50 of these cell lines used in all experiments in this section except where otherwise indicated; PRKDC knockout strongly synergized with doxorubicin in BE2C, but not GIMEN, in the CRISPR screen).