We therefore derived Gp130FF tumor organoids and used CRISPR/Cas9-mediated gene editing to impair Yap1 expression by co-transfecting organoids with the tracrRNA containing an ATTO550 label and the guide crRNA that targets exon 1 of the mouse Yap1 gene (Fig S5A and B). The gene discussed is YAP1; the disease is neoplasm.