Fecal microbiota:- ↑ Bifidobacterium (day 14 and day 42), lactic acid bacteria (day 42) and Clostridium (day 42).Hindgut fermentation products (fecal):- ↑ pH (day 28).Obesity:- ↓ Serum lipid profile (triglyceride, cholesterol) (day 42).Kidney and liver:- ↑ ALT (day 14), AST (day 28 and 42), and urea serum levels. Here, GPT is linked to obesity due to melanocortin 4 receptor deficiency.