Finally, we further conducted enrichment analysis on the genes in cluster 2 and found that TNFA signaling via NFkB and cytokine-cytokine receptor interaction pathways were the most significantly enriched (Figure 8(e)), indicating that these two pathways might be crucial for KYNU(+) macrophages to exert their procancer effects in GC. This evidence concerns the gene NFKB1 and gastric cancer.