tRF-21-VBY9PYKHD derived from tRNAGlyGCC could directly bind to Ser52 of hnRNP L, preventing the phosphorylation of hnRNP L by AKT2/AKT1, thereby inhibiting the formation of hnRNP L-DDX17 complex, ultimately inhibiting the invasion ability of cancer cells and promoting their apoptosis. The gene discussed is AKT1; the disease is cancer.