Therefore, in order to fill the gaps in previous studies, in this study, we observed the proliferation, invasion and apoptosis of DLBCL cell lines by overexpressing or knocking down the expression of miR-525-5p and Myd88, and constructed transplanted tumor nude mice overexpressing miR-525-5p to verify the regulatory role of miR-525-5p and Myd88 in the progression of DLBCL, in order to provide new ideas for the treatment of DLBCL. This evidence concerns the gene MYD88 and neoplasm.