More importantly, to demonstrate the endogenous p53R273H effects on the glucose metabolism, we perform the HT-29 NC or shp53 cells to co-culture Jurkat cells and the results showed that Jurkat cells in HT-29 shp53 group promotes the expression of p-PKM2 Y105, HK I and PFKP (Fig. 5G).Thereafter, to solidate the hypothesis that the secretion of mutant p53 affects the metabolism change of Jurkat, we took two strategies to inhibit the secretion of p53R273H by the means of performing the p53R273H LL25AA plasmid or knocking down the AP1B1 using siRNA in tumor cells. This evidence concerns the gene HK1 and neoplasm.