Besides, according to the results demonstrated in volcano plot, several top ranking genes (e.g. Drp2, Tnnt2, Myh4 and LDB3) were relevant to the top ranking processes like cardiac muscle contraction and hypertrophic cardiomyopathy (Fig. 6b and Supplementary Table S6), further q-PCR assay confirmed that these top ranking genes were also downregulated by si-PRMT1, indicating the importance of PRMT1 in regulating cardiac muscle contraction and hypertrophic cardiomyopathy in pathological cardiac remodeling (Fig. 6c). The gene discussed is MYH4; the disease is hypertrophic cardiomyopathy.