Sarco-spheres were challenged with a 78 pan-cancer drug library consisting of drugs assigned to ten categories: protein tyrosine kinase inhibitors (TKI), cell cycle/DNA damage regulators, epigenetic modifiers, MAPK/ERK signaling inhibitors, PI3K/AKT/mTOR inhibitors, metabolic enzyme and proteasome regulators, apoptosis modulators, JAK/STAT pathway modifiers and drugs active in the WNT pathway (Supplementary Table 4). This evidence concerns the gene AKT1 and cancer.