Using mass spectrometry analysis of MGC-803 cell and bioinformatic analysis, we identified phosphorylation of VPS35 was enhanced in GC cells, and phosphorylated VPS35 has enhanced interaction with ITGB3.Therefore, the integrin-FAK-SRC axis mediates YAP dependent IL-6/STAT3 signalling and is responsible for the VPS35-mediated promotion of GC proliferation and metastasis. This evidence concerns the gene PTK2 and gastric cancer.