Western blot analysis showed that the expression of related proteins, such as CDK4, CDK6, cyclin D1, and phosphorylated Rb (Ser807/811), was markedly increased in VPS35-overexpressing GC cells and decreased in VPS35-knockdown GC cells versus their control cells (Fig. 3K, L and Supplementary Fig. 3A, B). This evidence concerns the gene RB1 and gastric cancer.