They are less abundant in most cultured cells, as shown here in our EM analysis of COS-7 cells, but were found to be abundant by EM analysis of HT-1080 fibrosarcoma cells (Wang et al., 2015) that express robust amounts of the Gp78 ubiquitin ligase (Alan et al., 2022; Tsai et al., 2007). This evidence concerns the gene AMFR and fibrosarcoma.