The rationale behind the overexpression of SPRYD7 in CRC cells was to try to determine whether SPRYD7 overexpression would increase the tumorigenic and metastatic properties of KM12C, KM12SM, SW480, and SW620 cells, whereas the rationale behind the use of KM12C and KM12SM CRC cells for in vivo assays was to survey changes in liver homing metastasis and tumor growth and to determine whether SPRYD7 could induce poorly metastatic cells to become metastatic to the liver. This evidence concerns the gene SPRYD7 and neoplasm.