WB analyses with protein extracts from mock and SPRYD7-stably overexpressing transfected KM12C, KM12SM, SW480, and SW620 CRC cells confirmed the upregulation of KDELC2, SETD8, and DDB2, and the downregulation of IRF9 in CRC cells overexpressing SPRYD7, thus confirming the modulation of the protein expression levels of the analyzed proteins via SPRYD7 overexpression (Figure 5C). Here, DDB2 is linked to colorectal carcinoma.