Neither the staining pattern, nor the identification of the target-autoantigens have any specific clinical implication in patients with AIH.26 However, PBC-specific ANAs should deserve a special mention in screening that correspond to rim-like nuclear membranes and multiple nuclear dots on IIF using HEp-2 as substrate and gp210 and sp100 antigens in ELISA, with a sensitivity of 30% and specificity of 99%.34,35 Their presence warrants investigation for PBC/AIH variant syndromes in AIH patients with cholestatic features. This evidence concerns the gene SP100 and primary biliary cholangitis.