To further explore whether CARM1 decreases ACSL4 protein expression, we transfected different CARM1 overexpression plasmids into CRC cells and found that overexpression of wild‐type (WT) CARM1, but not the CARM1 R168A mutant deficient in methyltransferase activity,[29] significantly decreased ACSL4 protein levels (Figure 4h, Figure S4d, Supporting Information). This evidence concerns the gene ACSL4 and colorectal carcinoma.