To further evaluate the critical roles of ACSL4 methylation in ferroptosis in colon cancer in vivo, LoVo‐ACSL4‐KO, LoVo‐ACSL4‐WT, and LoVo‐ACSL4‐R339A cells were used to construct a mouse xenograft model. Here, ACSL4 is linked to malignant colon neoplasm.