To further investigate the mechanism of the upregulation of Srg3 in ALI, we found that Irf7 could bind to the Srg3 promoter and promote its transcriptional activation, thereby increasing the expression level of Srg3. In subsequent experiments, it was found that the symptoms of ALI in rats were significantly alleviated after treatment with Irf7-specific inhibitor or NF-κB signaling pathway-specific inhibitor. The gene discussed is NFKB1; the disease is acute respiratory distress syndrome.