To further examine the impact of the loss of CHEK2 on β-cell function in T2D condition, NLuc-EndoC-βH1 cells carrying control scrambled sgRNA or sgCHEK2 were cultured in the presence of 2 mM sodium oleate for 72 h and then assessed for their insulin secretory response to glucose. This evidence concerns the gene INS and type 2 diabetes mellitus.