Here we report, for the first time, the expression profile of the Orai family members, Orai1-3, and different TRPC channels, TRPC1, 3 and 6, in breast cancer stem cells (BCSC) derived from the widely used estrogen receptor-positive (ER+) cell line MCF7, the HER2 cell line SKBR3 and the triple negative breast cancer (TNBC) cell line MDA-MB-231, as well as in breast stem cells (BSC) derived from the non-tumoral breast epithelial cell line MCF10A. Here, ORAI1 is linked to triple-negative breast carcinoma.