We evaluated the genetic polymorphisms of TLR1/2, namely, TLR1 (N248S/rs4833095), TLR1 (S602I/rs5743618), TLR2 (N729S/rs61735278), TLR2 promoter (− 196 to − 174 deletion/rs111200466), TLR4 (D299G/rs4986790) and TLR4 (T399I/rs4986791), in stage II–III COAD patients (n = 869). This evidence concerns the gene TLR1 and colon adenocarcinoma.