PDCD1 and neoplasm: Here, we show that DTIC-laden Epacasome has stronger tumour inhibition over co-administration of DTIC/Lipo-SM/Chol and Epacasome, and it further boosts the anti-PD-1 therapy with complete metastasis remission in a late-stage melanoma mouse model by fortifying the NKs and CTLs responses.