In the next step, to investigate the diagnostic value of hub genes, which were significant in overall survival analysis, we applied ROC analysis on TCGA data of colon and rectal cancer—obtained AUC values of hub-genes (ORC1 AUC: 0.820, CDC6 AUC: 0.933, SMC4 AUC: 0.758, GINS2 AUC: 0.842, FANCI AUC: 0.930, CHEK1 AUC: 0.915, RACGAP1 AUC: 0.915, and MAD2L1 AUC: 0.897) proper diagnostic performance of hub genes in colorectal cancer (Fig. 9). This evidence concerns the gene CDC6 and colorectal cancer.