A near-diploid neuroblastoma cell line derived from a male infant, SK-N-BE(2), was transfected with a Cas9 nuclease disrupting the boundary of L1CAM intron 25 and exon 26 (ex26-1), and stained using an anti-L1CAM monoclonal antibody (5G3) and Alexa Fluor 488-conjugated secondary antibody at 4 days after transfection. This evidence concerns the gene L1CAM and neuroblastoma.