Two human hepatocellular carcinoma lines that support HEV replication (Huh7 and the derivative cell line Huh7.5, which contain a RIG-I mutation and support enhanced replication of viruses such as HCV [33, 34]) were transfected with an HEV-1 SGR RNA (SK-E2-nLuc), derived from the Sar55 infectious clone sequence [26] (Fig. 1a). Here, RIGI is linked to hepatocellular carcinoma.