PLAT and Sepsis: The optimal combination (sequential vs. concurrent) and frequency of intrapleural therapy are not well established. The landmark study on sequential therapy, Multicenter Intrapleural Sepsis Trial (MIST2), was conducted by Rahman and colleagues [2], wherein 210 patients with pleural infections were randomly assigned to four study groups, one group being given sequential intrapleural tPA and DNase twice a day for three days.