We observed that secretions from IFN-β-stimulated CF AECs enhanced P. aeruginosa biofilm formation compared to that of the unstimulated control, with a reduction in P. aeruginosa biofilm biomass when IFN-β-stimulated CF AECs were treated with 2-DG or NaOx (Fig 4A). This evidence concerns the gene IFNB1 and cystic fibrosis.