To select peptides with the highest potential as putative immunotherapeutic targets, we prioritized peptides on the basis of pMHC complex binding affinity, HLA allele frequency, degree of differential expression of parent genes, relative abundance on MHC compared with other peptides, recurrence across multiple neuroblastoma tumours and relevance to neuroblastoma biology based on the published literature23–25. This evidence concerns the gene HLA-C and neoplasm.