The improved cytotoxicity of Vδ2 T cells expanded from CD16Hi donors correlated with increased IFN-γ secretion, as measured by ELISA, and perforin and granzyme B production, as measured by intracellular staining, following 24 h (h) co-culture of cancer cells and effector cells at a 1:1 E:T ratio in the presence or absence of ZOL (Fig. 1h). The gene discussed is PRF1; the disease is cancer.