Differences between healthy donors (3 day) and melanoma patient mDC (5 days) iDC cultures do not impact our results as studies examining differences in immature iDC generation revealed that monocytes cultured in the presence of IL-4 + GM-CSF within 48 h exhibit iDC characteristics and upon maturation these cells displayed a fully mature mDC immunophenotype38. This evidence concerns the gene CSF2 and melanoma.