Transcripts for HCV entry factors including cluster of differentiation 81 (CD81), occludin (OCLDN), claudin-1 (CLDN1), and scavenger receptor class B type I (SR-B1) were more highly expressed in organoids than in primary human hepatocytes with no consistent differences associated with infection status and at similar levels before and after differentiation (Fig. 1C). This evidence concerns the gene CD81 and infection.