However, TRIM21 levels were profoundly increased in livers of obese mice on a high-fat diet (HFD, 60% fat) or a NASH diet (40% fat, 30% fructose diet, and 2% cholesterol) as compared with ND controls (Supplemental Figure 2A and Figure 2, A–C). Here, TRIM21 is linked to metabolic dysfunction-associated steatohepatitis.