To further confirm the function of TRIM21 in hepatocytes and in NASH pathogenesis, we knocked down TRIM21 by approximately 60% in livers of mice fed a NASH diet for 30 weeks using a recombinant adenovirus-mediated delivery of shTrim21 for 9 days (Figure 6, A and B). The gene discussed is TRIM21; the disease is metabolic dysfunction-associated steatohepatitis.