Therefore, although our Trim21 overexpression data in vivo and in vitro indicate that ChREBP is a direct target for degradation, our transient sh-mediated knockdown data cannot conclude that ChREBP acts in synergism with SREBP1 to increase steatosis; we thus hypothesize that the exaggeration of steatosis is mainly due to the activation of SREBP1 and FASN. This evidence concerns the gene FASN and steatosis.