Feeding mice a high-fructose diet (60% fructose for 8 weeks) led to a modest (approximately 1.8-fold) increase in TRIM21 expression, which was amplified in mice fed a high-fat and high-cholesterol diet containing fructose (NASH diet) (Figure 2, A–C). This evidence concerns the gene TRIM21 and metabolic dysfunction-associated steatohepatitis.