Transient hepatic Trim21 silencing resulted in a moderate rise in inflammatory (TNF-α, F4/80) and fibrosis (Col1a1) markers, consistent with elevated steatosis, though it resulted in no changes in αSMA expression, JNK/TAK1 signaling, or plasma ALT levels in livers of NASH mice in which Trim21 was silenced compared with controls (Supplemental Figure 6, F–K). This evidence concerns the gene COL1A1 and steatosis.