Moreover, in KRAS-mutant CRC cells, supplementation with α-KG or additional Gln in Gln-containing full medium rescued the decrease in proliferation and migration mediated by SLC25A21 overexpression, whereas SUCLG2 downregulation abolished the rescue effect of Gln or α-KG supplementation in culture medium on the proliferation and migration of SLC25A21-overexpressing cells, but not KRAS-WT CRC cells (Figure 5G and Supplemental Figure 8B). The gene discussed is SUCLG2; the disease is colorectal carcinoma.