To further assess the growth suppression mediated by SLC25A21, we transfected the LV5-SLC25A21 vector into 3 human primary CRC organoids and found that SLC25A21 overexpression suppressed organoid growth in Matrigel from CRC with KRAS mutation; however, SLC25A21 overexpression in KRAS-WT CRC organoids did not alter their growth in vitro (Figure 2E). The gene discussed is KRAS; the disease is colorectal carcinoma.