SLC25A21 and colorectal carcinoma: As predicted, in both KRAS-mutant and KRAS-WT CRC cells, SLC25A21 knockdown led to an accumulation of α-KG in mitochondria (P < 0.05); however, SLC25A21 depletion only increased the mitochondrial α-KG content by 22% in KRAS-WT cells (Figure 4A).